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TBST buffer

From Molecular Biology Wiki

TBST is commonly used as a (nitrocellulose, nylon, PVDF) membrane wash buffer during Western Blot.


TBS-T 1X Buffer Recipe

To Make 1L of TBST add the following:

  • 100 ml 10x TBS
  • 10 ml 10% Tween20 (final 0.1% v/v)
  • dissolve with 890 ml double distilled-H2O (ddH20)
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