In molecular biology, TBE and TAE are often used in procedures involving DNA, the most common being electrophoresis. Tris is an effective buffer for slightly basic solutions, which keeps DNA deprotonated and soluble in water. EDTA binds to divalent cations, particularly magnesium (Mg2+). These ions are necessary co-factors for many enzymes; Magnesium is a co-factor for many DNA-modifying enzymes.
Tris Borate EDTA (TBE) suppliers include:
American Bioanalytical www.americanbio.com
â†‘ Yutaka MIURA, Hiroaki WAKE, and Taiji KATO TBE, or not TBE; that is the question: Beneficial usage of tris-borate for obtaining a higher resolution of small DNA fragments by agarose gel electrophoresis. Nagoya Medical Journal 43(1) 1-6, 1999