Abscisic acid (4,818 bytes) 1: == Chemical Nature pf Abscisic Acid == 4: ... referred to as ABA is synthesized from melavonic acid. Sites of Biosynthesis: In mature leaves, especia... 20: ...l function and gene expression. -PA (Phosphatidic acid) produced by PLD mediates also the regulation of ...
Nucleic acid (2,979 bytes) 1: == Nucleic Acids a History of Discovery == 3: The nucleic acids which were principally studied from the early 19... 6: ...osine ; a hextose (carbohydrate) ; and phosphoric acid. 12: ... fatty acid radicles, such as stearic acid, oleic acid, etc., and a nitrogenous base (cholin). It
Acetonic acid (201 bytes) 1: ...],formed by the union of [[acetone]] and [[formic acid]].
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DNA (13,310 bytes) 7: == DNA - DeoxyriboNucleic Acid == 9: ...oNucleic Acid, or usually 2'-deoxy-5'-ribonucleic acid. 11: Deoxyribonucleic acid (DNA) is a nucleic acid which carries the genetic instructions for the bi... 79: ...d. Since there are 64 possible codons, most amino acids have more than one possible codon. There are als... 99: Nucleic acids absorb light at this wavelength because of the e...
RNA (14,087 bytes) 1: ... 3-bases in an mRNA sequence each encode an amino acids, which when synthesis in tandem with other 3-bas... 5: Nucleic acids were discovered initially from pus in open wound... 7: ...Miescher separated nucleins into a protein and an acid molecule in 1874. 9: ...which do not have a nucleus, also contain nucleic acids. 30: ...nly consistent difference between the two nucleic acids, that distinguishes it from DNA is the presence ...
Protein (8,861 bytes) 1: ...d of [[amino acids]]. The sequence of the [[amino acid]]s, and thus the function of the protein, is dete... 26: ...ffinity for acid stains like eosin are said to be acidophile or oxyphile ; those showing affinity for ba... 28: ...taining nucleic acid, the latter being an organic acid, rich in phosphorus ; it was hence called nucleop... 31: ...c alkali it decomposed into protein and [[nucleic acid]]. 38: ...e surface representation colored by residue type (acidic residues red, basic residues blue, polar residu...
Dna replication (5,207 bytes) 13: ...Nucleic acid nomenclature|5’]] to [[Nucleic acid nomenclature|3’]] (of the new DNA). Because... 46: ... incorporation of [[deoxyribonucleotide]]s into [[acid]] or [[ethanol]] insoluble forms.
Electrophoretic mobility shift assay (5,322 bytes) 12: ...ding to and interacting with a DNA or RNA nucleic acid sequence fragment. Denaturing EMSAs are done by U... 13: Thus, you theoretically should result in nucleic acid fragments that are bound only to directly interac... 17: ...es of complexes that bind to a DNA or RNA nucleic acid sequence fragment. Non-denaturing EMSAs are condu...
DNA EMSA protocol (835 bytes) 23: * 44.5 mM [[Boric acid]] 24: * 1 mM [[EDTA]] (free acid)
TBE (1,465 bytes) 1: ...on]] that consists of [[Tris|Tris base]], [[boric acid]], [[EDTA]], and [[water]].
Polymerase chain reaction (44,952 bytes) 32: ...quence corresponding to the codon for the [[amino acid]] [[isoleucine]] may be "ATH", where A stands for... 172: ...expression|gene's expression]]. [[RNA|Ribonucleic acid (RNA)]] is the molecule into which DNA is transcr...
Primer (8,561 bytes) 1: A '''primer''' is a [[nucleic acid]] strand, or a related [[molecule]] that serves a... 28: ...fore primer sequence corresponding to the [[amino acid]] [[isoleucine]] might be "ATH", where A stands f... 33: ...fore primer sequence corresponding to the [[amino acid]] [[isoleucine]] might be "ATH", where A stands f...
DNA replication (5,554 bytes) 20: ...Nucleic acid nomenclature|5’]] to [[Nucleic acid nomenclature|3’]] (of the new DNA). Because... 61: ... incorporation of [[deoxyribonucleotide]]s into [[acid]] or [[ethanol]] insoluble forms.
Deoxyribonucleotide (859 bytes) 3: ..., or single unit, of [[DNA]], or deoxyribonucleic acid. Each deoxyribonucleotide is comprised of three p...
Agar plates (9,552 bytes) 51: ...l. If mannitol fermentation is occurring, lactic acid will be produced, and the pH will drop causing th...
Gibberlin (5,314 bytes) 6: ...ly available GA. GA is synthesized from mevalonic acid. GA biosynthesis inhibitor: paelobutrazol (PAC) o...
Abscisic acid (4,818 bytes) 1: == Chemical Nature pf Abscisic Acid == 4: ... referred to as ABA is synthesized from melavonic acid. Sites of Biosynthesis: In mature leaves, especia... 20: ...l function and gene expression. -PA (Phosphatidic acid) produced by PLD mediates also the regulation of ...
SDS-PAGE (17,204 bytes) 12: ...mary structure]], or number (and size) of [[amino acid]]s). The SDS binds to the protein in a ratio of ... 26: ...s an innocuous substance to most proteins. Its [[Acid dissociation constant|pKa]] is 8.3 at 20 °C and ... 28: * '''[[Glycine]] (Amino Acetic Acid) (C2H5NO2; mw: 75.07).''' Glycine has been used a... 44: ...ecule it moves ahead of most proteins and nucleic acids. As it reaches the [[anode|anodic]] end of the ... 48: ... (7%).''' Proteins in the gel are fixed by acetic acid and simultaneously stained. The excess dye incor...
SDS (8,977 bytes) 51: ...at a ratio of one SDS anion for every two [[amino acid]] residues. This effectively imparts an negative ...
EDTA (5,199 bytes) 11: ...c acid<br>Edetate<br>Ethylenedinitrilotetraacetic acid<br>Versene 51: ...chemical compound]] '''ethylenediaminetetraacetic acid'''. 55: EDTA features four [[carboxylic acid]] and two [[amine]] groups that can all bind to m... 61: ...ligands are all formally derived from the [[amino acid]] [[glycine]]. 105: * [[Nitrilotriacetic acid|NTA]]
10X TAE buffer (318 bytes) 12: * Adjust pH to 7.6 with glacial acetic acid CH3COOH or tris
10X TBE buffer (258 bytes) 8: * 0.89 M Boric Acid 10: * pH 8.3 with boric acid or [[Tris]]
