Video shown below of an SDS-PAGE running and separation shown at a fast speed.
Gel electrophoresis is a technique in which an electric current is used to move charged molecules through a gel-like matrix. The direction, distance, and speed of migration are dependent on the size, shape and electrical charge of the molecules and the pore-size of the gel matrix. Gel electrophoresis is routinely used to separate proteins or nucleic acids; specific protocols have been developed for each type of molecule. Common gel materials are agarose (a polysaccharide) and acrylamide (a 3-carbon amide which is polymerized to form long chains with cross-links between the chains). The pore size of the gel is influenced by the percentage of gel material used and, in the case of acrylamide, the amount of cross-linking.
PAGE (polyacrylamide gel electrophoresis) is commonly used to analyze protein samples.
SDS-PAGE is a denaturing separation method, since it uses SDS a denaturing detergent.
Materials Needed for SDS-PAGE
Molecular Weight Marker
4-20% acrylamide gradient gels
Tris-glycine-SDS buffer
Practice gel loading solution
Marker protein
Sample proteins
Sealant
50 ml Coomassie Blue Staining Solution
De-staining solution (7.5% acetic acid)
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