Running buffer is a buffer that is made to separate proteins in a gel matrix. It is commonly made for SDS-PAGE gels to separate proteins.
1X running buffer is commonly prepared by diluting 10X running buffer into 1X.
For example, dilute 100 mL of 10X running buffer with 900 mL of Milipore or double distilled water (ddH20).
10X running buffer
For more click here:Science News