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ViewsRNA visualizationFrom Molecular Biology WikiRNA or ribonucleic acid may be visualized on agarose gels or polyacrilamide gels using ethidium bromide staining or SYBR dyes which are much safer. See also RNA Gel Electrophoresis and Gel electrophoresis.
Ethidium Bromide Staining of RNA Agarose GelsEthidium bromide (EtBr) is added to the gels not the running buffer. This is a safety precaution as ethidium bromide is mutagenic. Add 5ul from 10mg/ml stock of EtBr per 100 mL of 1X (or 0.5X depending on your apparatus) TAE or TBE buffer.
SYBR Visualization of RNAIf you are using SYBR Green, SYBR Orange or other SYBR staining you usually incubate your agarose gel after running in 5ul of SYBR with 50mL of 1X TAE or TBE buffer for 5-20 minutes. See manufacturer's instructions for more information! RNA Visualization References
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