RNA protocols

From Molecular Biology Wiki

RNA protocols are methods and procedures used for the analysis of RNA.

Working with RNA

RNA is not as stable as DNA as it is usually single-stranded, and also is digested by RNAses which are quite abundant in the environment. For this reason, RNA work is usually conducted in a sterile environment or sterile tubes, DEPC treated water and buffers, and also RNAse inhibitors.

• See also: DEPC Buffer

RNA Techniques Protocols

• Northern blot protocol

RNA Gel and Analysis Protocols

• RNA electrophoresis
• RNA visualization on gels.
• RNA analysis
• RNA quantitation
• RNA gel protocol

RNA Extraction Protocols

• RNA extraction
• RNA extraction protocol

More RNA Protocols

Go to RNA Protocols for a complete listing of RNA protocols from hundreds of other laboratories.

RNA Forum Discussion

Join the specifc discussions on RNA! Or simply click on the top (Discussion) link to talk in general about PCR!

		Thread / Thread Starter	Last Post	Replies	Views	Forum
		Micromanipulation of Gene Expression in the Adult Zebrafish Brain Jon Moulton	Yesterday 07:14 PM by Jon Moulton	0	29	RNA Techniques Forum
		Vivo-Morpholinos Induced Transient Knockdown of Physical Activity Related Proteins Jon Moulton	05-02-2013 10:08 PM by Jon Moulton	0	56	RNA Techniques Forum
		Microinjecting mRNA or morpholinos in the starlet sea anemone, Nematostella vectensis Jon Moulton	04-15-2013 04:31 PM by Jon Moulton	0	86	RNA Techniques Forum
		Antihypertensive Actions of Moderate Hyperbilirubinemia (mouse knockdown) Jon Moulton	03-14-2013 06:47 PM by Jon Moulton	0	101	RNA Techniques Forum
		The miR-183/Taok1 Target Pair Is Implicated in Cochlear Responses to Acoustic Trauma Jon Moulton	03-06-2013 10:42 PM by Jon Moulton	0	119	RNA Techniques Forum
		Circular RNAs are a large class of animal RNAs with regulatory potency Jon Moulton	03-05-2013 02:51 PM by Jon Moulton	0	131	RNA Techniques Forum
		Identification of Nonvisual Photomotor Response Cells in the Vertebrate Hindbrain Jon Moulton	02-27-2013 07:32 PM by Jon Moulton	0	113	RNA Techniques Forum
		Trapping Cardiac Recessive Mutants via Expression-Based Insertional Mutagenesis Scrn Jon Moulton	02-20-2013 06:15 PM by Jon Moulton	0	114	RNA Techniques Forum
		Alternative splicing or non-optimal PCR conditions? Montojo	01-25-2013 03:39 PM by Jon Moulton	1	188	RNA Techniques Forum
		Antisense oligonucleotides (ASOs) and ss-siRNA Alleunram	01-08-2013 07:44 PM by Jon Moulton	1	177	RNA Techniques Forum