When preparing buffers:
Using Milipore Water Destillation or Water deionization units for Buffers:
When using millipore (or other purifying units) water, make sure you only use H2O with a resistance of at least 18 MOhm/cm.
To autoclave a 0.5 L (liter) solution you should autoclave for 15 minutes at 121Â°C at 1 bar.
To autoclave a 1 liter solution you should autoclave for 20 minutes at 121Â°C at 1 bar.
NOTE: You should NOT autoclave buffers containing:
In this case, filter sterilize solutions through a 0.22 Âµm filter as an alternative!
See Autoclaving for more information.
See pH measurement
See preparing RNA buffers and solutions
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