Special Feature

My Panel

Bookmark Science Articles

Recent News

Pcr master mix

From Molecular Biology Wiki

What is a PCR master mix?

PCR Master Mix Definition

A PCR master mix is a mix of all most of the ingredients or factors necessary for PCR to occur. The goal of a PCR master mix is to decrease time spent pipetting.


How a PCR Master Mix Works

A PCR master mix works similar to the following:

If you had to make 10 recipes that were similar except for 1 ingredient that was different in each (usually in a PCR master mix the DNA template), you could simply make a "master mix" of 11X all the ingredients (1 extra in case of lost recipe). You could then add 1X of the mix to the 10 pots, and then add that extra ingredient to each of the 10 pots.

Pcr master mix
 

Wiki Menu

Science Wiki

Protocols

DNA Forum

Science Forum

DNA Forum
Biology Forum

Science News

For more click here:Science News