PBS

From Molecular Biology Wiki

PBS - Phosphate Buffered Saline

Applications of PBS

PBS is a buffer commonly used to wash cells or western blot membranes.

Storage of PBS Solution

PBS is stable at room temperature, however can also be stored at 4 degrees C.

20X PBS stock solution gives a longer shelf life than 1X or even 10X.

PBS Stock Solution Buffer Recipe (1 L)

• Dissolve in 800 mL of Water H20:
• 80 g of NaCl
• 2 g of KCl
• 26.8 g of Na2HPO4 - 7 H20
• 2.4 g of KH2PO4
• Adjust pH to 7.4 with 1M HCl
• Adjust volume to 1 L with Water