I am interested in normal blood flora discovery in Bulgaria.Chlamydia-like microorganisms are living in donors blood as normal flora and they can be isolate if 0.5 ml donors blood is inoculate in 4.5 ml brain hearth infusion with 0.25% sodium citrate.After 14 days incubation at 43 grad C a Gram stained slide from the sediment demonstrate the new microorganisms inside the erythrocite as in nests.PCR is impossible because the new microorganisms are resistant to destruction.
I will be very grateful if some scientist can do PCR for the first time in microbiology.More information in Google, Kalfin E
E Kalfin M.D.Ph.D
