DNA EMSA protocol

From Molecular Biology Wiki

Overview of EMSA Procedure

Function of the EMSA is that it is an assay which checks for the presence of protein-DNA binding.

Materials Needed to Perform DNA EMSA

DNA EMSA Electrophoresis

Loading solution

• It is usually light sensitive so store in dark area (cover with aluminum foil)
• stored at -20°C
• comes in EMSA kits from Pierce (Light Shift Chemiluminescent Kit) and Invitrogen (manual, catalog number - E-33075)
• Typical DNA gel loading buffer can also be used.

Running Buffer for Electrophoresis

0.5X TBE running buffer

• 44.5 mM Tris base
• 44.5 mM Boric acid
• 1 mM EDTA (free acid)
• pH usually around 7-8
• You can make yourself or buy from a supplier such as Invitrogen (catalog number LC6675)

'DNA ladder for mobility shift assay'