DEPC-treated water

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DEPC Treated Water

DEPC Treated Water is used when you want to prevent RNase or DNase contamination.

DEPC Treated Water Recipe

- Add 1 mL of 0.1% Diethylpyrocarbonate (DEPC) to 1000 L of distilled H2O

- Mix and leave at room temperature for 1 hour.

- Autoclave

- Cool to room temperature prior to use. Additional comments : DEPC water (H20) is used for RNA protocols.