Cell lysis buffer

From Molecular Biology Wiki

Cell lysis buffer

Cell lysis buffer is buffer for the lysis of cells.

Non-Denaturing Cell lysis buffer

1X Cell Lysis Buffer Non-Denaturing

- 20 mM Tris-HCl pH 7.5 - 150 mM NaCl - 1 mM Na2EDTA - 1 mM EGTA - 1% Triton - 2.5 mM sodium pyrophosphate - 1 mM b-glycerophosphate - 1 mM Na3VO4 sodium ortho-vanadate - 1µg/ml leupeptin

Additional comments : This cell Lysis Buffer purpose is to lyse cells under non-denaturing conditions.

Cell Lysis Buffer Non-Denaturing Alternate 1X

- 10mM imidazole pH 7.3 - 0.5 M NaCl - 1% Triton X-100 - 2mM sodium azide - 0.2mM sodium ortho-vanadate - 0.2mM PMSF Additional comments : Store at -20°C

Used to lyse cells under non-denaturing conditions.

Denaturing Cell lysis buffer