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10X TBST buffer

From Molecular Biology Wiki

Stock solution for TBST buffer. Commonly used in western blot as a membrane wash solution.


10X TBST Buffer Recipe

To make 10x TBST (wash buffer), add the following together:

  • 12.11 g Tris
  • 87.66 g NaCl
  • 5 mL Tween-20
  • 1 g Na-azide

Bring up the mix with 1 liter ddH20 distilled water


To make 1x TBST, add 100 mL of 10x TBST to 900 mL of distilled water to make 1L 1X solution.

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