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10X TAE buffer

From Molecular Biology Wiki

10X TAE buffer is a stock solution for TAE.


10X TAE Buffer Preparation

To prepare 10X TAE you need to add the following:

  • 0.4 M Tris-base
  • 11.4% (v/v) glacial CH3COOH
  • 10 mM EDTA (disodium)
  • Adjust pH to 7.6 with glacial acetic acid CH3COOH or tris


see also TAE

10X TAE buffer
 

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