10X MOPS

From Molecular Biology Wiki

10X MOPS buffer is the stock solution for 1X MOPS buffer.

10X MOPS Buffer Recipe Preparation

To prepare 10X MOPS add the following:

Add 41.85 g 4-morpholinopropanesulfonic acid (MOPS – free acid, MW = 209.27) and 6.80 g sodium acetate – 3H2O (NaOAc – 3H2O, MW = 136.08) to 800 ml DEPC treated H2O and stir until completely dissolved.

• Add 20 ml of a DEPC treated 0.5 M Na2EDTA solution and adjust pH to 7.0 with 10 M NaOH or Glacial acetic acid CH3COOH.

• Adjust volume to 1 liter with DEPC treated H2O.

Storage of 10X MOPS

Divide into 200 ml aliquots and store – protected from light – at 4°C. NOTE: If the solution turns yellow, use a new aliquot.