10X MOPS buffer is the stock solution for 1X MOPS buffer.
To prepare 10X MOPS add the following:
Add 41.85 g 4-morpholinopropanesulfonic acid (MOPS â€“ free acid, MW = 209.27) and 6.80 g sodium acetate â€“ 3H2O (NaOAc â€“ 3H2O, MW = 136.08) to 800 ml DEPC treated H2O and stir until completely dissolved.
Divide into 200 ml aliquots and store â€“ protected from light â€“ at 4Â°C.
NOTE: If the solution turns yellow, use a new aliquot.
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