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Stem cell culture without feeder cell
I have been doing experiments with HESCs, H1 and H9 (from national stem cell bank). Right now, I am co-culturing the cell lines with feeder cell line...
Dexametasone
Hi
i am working on differenciation of mesenchymal stem cells into osteoblasts. will anybody guide me that whether dexamethasone is essential for...
Pipette Question
Dear Everyone,
Does anyone know where to buy the mouth piece of the mouth pipette? Or if anyone knows any vendor sells a mouth pipette set? I...
viral vector
Can anyone tell me the protocols of transfecting stem cells with viral vetors.
Low Homologous Recombination Rates Stem Cells
Hello, i am new to the gene targeting gane and to this amazing forum also
I am conducting gene targeting experiments using mouse embyronic ES stem...
plasmid transfection
Hello! Hope this is the right place to post this...
I'm working on a project with mouse stem cells, mainly 46C, and one of the experiments seems to...
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What is a stem cell? A cell that is capable of proliferation, self-renewal and differentiation into at least one cell type. These are the minimum properties of a stem cell.
Self renewing: They are able to give rise to another cell type and this other cell type will expand the population but not require expansion itself. Eventually this cell type will need by definition to differentiate into a specific cell type. Determination: when start going down a specific lineage, cells that come off cell lineage begin to differentiate. All of them could be identical of different into capacities to differentiate into different cell types.
A stem cell can divide by 2 forms of division and the consequence of dividing by one of these modes of division will cause the stem cell to be different.
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