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Need help with specific lab calculations..
:help:Can someone please help me with the following question?:
"You are going to set up a 60 microlitre solution of enzyme in buffer. You want a...
Temperature Effect on Immunohistochemistry Signal Noise Ratio?
Hi guys, im currently working with a Immunohistochemistry protocol that has overnight incubations in with the primary antibodies for 3-5 hours at...
Low Luciferase Expression after Transfection of HEK293 Cells
Hello i have a big issue. I transfected my cells with a dual luciferase constructs such as RENILLA and FIREFLY LUCIFERASE
the issue is that the FF...
Dual Transfection Issue with Reporters
Hi guys, how can one transfect two constructs equally in situations of protein regulation (checking A regulation of B) and dual-reporters such as the...