Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis.

Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Research Abstract Details 

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Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Abstract Text:

Tinchung Leung,Hui Chen,Anna M Stauffer,Kathryn E Giger,Soniya Sinha,Eric J Horstick,Jasper E Humbert,Carl A Hansen,Janet D Robishaw,

Vascular endothelial growth factor (VEGF) is a major mediator of pathologic angiogenesis, a process necessary for the formation of new blood vessels to support tumor growth. Historically, VEGF has been thought to signal via receptor tyrosine kinases, which are not typically considered to be G protein dependent. Here, we show that targeted knockdown of the G protein gng2 gene (Ggamma2) blocks the normal angiogenic process in developing zebrafish embryos. Moreover, loss of gng2 function inhibits the ability of VEGF to promote the angiogenic sprouting of blood vessels by attenuating VEGF induced phosphorylation of phospholipase C-gamma1 (PLCgamma1) and serine/threonine kinase (AKT). Collectively, these results demonstrate a novel interaction between Ggamma2- and VEGF-dependent pathways to regulate the angiogenic process in a whole-animal model. Blocking VEGF function using a humanized anti-VEGF antibody has emerged as a promising treatment for colorectal, non-small lung cell, and breast cancers. However, this treatment may cause considerable side effects. Our findings provide a new opportunity for cotargeting G protein- and VEGF-dependent pathways to synergistically block pathologic angiogenesis, which may lead to a safer and more efficacious therapeutic regimen to fight cancer.

Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Publishing Authors By Initials

T Leung,H Chen,AM Stauffer,KE Giger,S Sinha,EJ Horstick,JE Humbert,CA Hansen,JD Robishaw,

For similar proteins: fish proteins: zebrafish proteins research abstracts see: proteins: fish proteins: zebrafish proteins research

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Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood

VOLUME: 108

Page Numbers: 160-6

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 14

MONTH: 03

YEAR: 2006

Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Keywords Mesh Terms:

KEYWORDS: Zebrafish Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Zebrafish G protein gamma2 is required for VEGF signaling during angiogenesis.

AFFILIATION: Weis Center for Research, Geisinger Health System, Danville, PA 17822, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM58191

ACRONYM: GM

MEDLINETA: Blood

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