Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Abstract Text:

Ke Liu,Zhaolin Hua,Joshua A Nepute,Todd R Graham,

Drs2p family P-type ATPases (P4-ATPases) are required in multiple vesicle-mediated protein transport steps and are proposed to be phospholipid translocases (flippases). The P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and here we define endocytosis signals required by these proteins to maintain a steady-state localization to internal organelles. Internalization of Dnf1p from the plasma membrane uses an NPFXD endocytosis signal and its recognition by Sla1p, part of an endocytic coat/adaptor complex with clathrin, Pan1p, Sla2p/End4p, and End3p. Drs2p has multiple endocytosis signals, including two NPFXDs near the C terminus and PEST-like sequences near the N terminus that may mediate ubiquitin (Ub)-dependent endocytosis. Drs2p localizes to the trans-Golgi network in wild-type cells and accumulates on the plasma membrane when both the Ub- and NPFXD-dependent endocytic mechanisms are inactivated. Surprisingly, the pan1-20 temperature-sensitive mutant is constitutively defective for Ub-dependent endocytosis but is not defective for NPFXD-dependent endocytosis at the permissive growth temperature. To sustain viability of pan1-20, Drs2p must be endocytosed through the NPFXD/Sla1p pathway. Thus, Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis. These results demonstrate an essential role for endocytosis in retrieving proteins back to the Golgi, and they define critical cargos of the NPFXD/Sla1p system.

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Publishing Authors By Initials

K Liu,Z Hua,JA Nepute,TR Graham,

For similar proteins: ubiquitins: ubiquitin research abstracts see: proteins: ubiquitins: ubiquitin research

PUBMED ID PMID:

MEDLINE DATE:

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 487-500

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 22

MONTH: 11

YEAR: 2006

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Keywords Mesh Terms:

KEYWORDS: Ubiquitin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Information

Substance Name: Calcium-Transporting ATPases

Registry Number: EC 3.6.1.8

Grant and Affiliation Information for Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.

AFFILIATION: Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235-1634, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-62367

ACRONYM: GM

MEDLINETA: Mol Biol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway Related Publications

• A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
• Assay of frozen boar semen with Sephadex filtration.
• Conceptual organization at 6 and 8 years of age: evidence from the semantic priming of object decisions.
• Current understandings of Helicobacter pylori, peptic ulcer and gastroesophageal reflux disease.
• Development of a buffer system for dialysis of bovine spermatozoa before freezing. I. Effect of zwitterion buffers.
• Development of a buffer system for dialysis of bovine spermatozoa before freezing. II. Effect of sugars and sugar alcohols on posthaw motility.
• Development of a buffer system for dialysis of bovine spermatozoa before freezing. III. Effect of different inorganic and organic salts on fresh and frozen-thawed semen.
• Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during the cryopreservation process.
• Effects of cryopreservation procedures on sperm membranes.
• Forty-four years of studying light adaptation using the probed-sinewave paradigm.
• Histological risk markers for non-cardia early gastric cancer. Pattern of mucin expression and gastric cancer.
• Importance of atrophic gastritis in diagnostics and prevention of gastric cancer: application of plasma biomarkers.
• Induction of DNA strand breaks and oxidative stress in HeLa cells by ethanol is dependent on CYP2E1 expression.
• Issues of scale and uncertainty in the global remote sensing of disease.
• Myth: Cerebrospinal fluid analysis can differentiate bacterial meningitis from aseptic meningitis.
• No evidence for the presence of apolipoprotein epsilon4, interleukin-lalpha allele 2 and interleukin-1beta allele 2 cause an increase in programmed cell death following traumatic brain injury in humans.
• PKR1 encodes an assembly factor for the yeast V-type ATPase.
• Partial k-space reconstruction in single-shot diffusion-weighted echo-planar imaging.
• Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane.
• Rs05 craft group audit for rural surgeons.
• Safety, immunogenicity and efficacy of modified vaccinia Ankara (MVA) against Dryvax challenge in vaccinia-naïve and vaccinia-immune individuals.
• Solitary coherent structures in viscoelastic shear flow: computation and mechanism.
• The global distribution of yellow Fever and dengue.
• The histopathology of fatal untreated human respiratory syncytial virus infection.
• The in vitro effect of boar semen on the contractility of rat uteri.
• Ultrastructure of the domestic tom cat (Felis domestica ) and tiger (Panthera tigris altaica ) spermatozoa.
• Visualization of the small intestine using capsule endoscopy in healthy subjects.
• Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.
• boc-Aspartyl(OMe)-fluoromethylketone attenuates mitochondrial release of cytochrome c and delays brain tissue loss after traumatic brain injury in rats.
• tRNA's wobble decoding of the genome: 40 years of modification.