[XBP-1 enhances the transcriptional activity of estrogen receptor alpha]

[XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Research Abstract Details 

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[XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Abstract Text:

The estrogen receptor (ERalpha) is a member of a large superfamily of nuclear receptors that regulates the transcription of estrogen-responsive genes. Several recent studies have demonstrated that human X-box binding protein 1 (XBP-1) mRNA expression is associated with ERalpha status in breast tumors. More recently, two forms of XBP-1 were identified due to their unique splicing. The two splicing variants of XBP-1 were designated XBP-1S and XBP-1U, respectively. In this study, the coding sequences of XBP-1S and XBP-1U were cloned respectively into the expression vector pcDNA3 harboring FLAG epitope, generating the recombinant plasmids pcDNA3-FLAG-XBP-1S and pcDNA3-FLAG-XBP-1U. Western blot analysis showed that both XBP-1S and XBP-1U were expressed in mammalian cells. To determine the effects of XBP-1S and XBP-1U on the transcriptional activity of ERalpha, MDA-MB-231 breast cancer cells were cotransfected with the expression vectors for ERalpha and either pcDNA3-FLAG-XBP-1S or pcDNA3-FLAG-XBP-1U. The results indicated that XBP-1S and XBP-1U enhanced ERalpha-mediated transcriptional activities in a hormone-independent manner. GST pull-down assay showed that both XBP-1S and XBP-1U interacted with ERalpha. These data suggest that XBP-1S and XBP-1U may play an important role in breast cancer growth and progression through ERalpha signaling.

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MEDLINE DATE:

[XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta bi

VOLUME: 35

Page Numbers: 829-33

Journal Abbreviation: Sheng Wu Hua Xue Yu Sheng Wu W

ISSN: 0582-9879

DAY: 5

MONTH: Sep

YEAR: 2003

[XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 20730160

[XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: [XBP-1 enhances the transcriptional activity of estrogen receptor alpha] Information

Substance Name: Glutathione Transferase

Registry Number: EC 2.5.1.18

Grant and Affiliation Information for [XBP-1 enhances the transcriptional activity of estrogen receptor alpha]

AFFILIATION: Beijing Institute of Biotechnology, Beijing 100850, China.

Country: China

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MEDLINETA: Sheng Wu Hua Xue Yu Sheng Wu W

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