X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase.

X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Research Abstract Details 

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X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Abstract Text:

Graham N George,Christian J Doonan,Richard A Rothery,Nasim Boroumand,Joel H Weiner,

Structural studies of dimethyl sulfoxide (DMSO) reductases were hampered by modification of the active site during purification. We report an X-ray absorption spectroscopic analysis of the molybdenum active site of Escherichia coli DMSO reductase contained within its native membranes. The enzyme in these preparations is expected to be very close to the form found in vivo. The oxidized active site was found to have four Mo-S ligands at 2.43 A, one Mo=O at 1.71 A, and a longer Mo-O at 1.90 A. We conclude that the oxidized enzyme is a monooxomolybdenum(VI) species coordinated by two molybdopterin dithiolenes and a serine. The bond lengths determined for E. coli DMSO reductase are very similar to those determined for the well-characterized Rhodobacter sphaeroides DMSO reductase, suggesting similar active site structures for the two enzymes. Furthermore, our results suggest that the form found in vivo is the monooxobis(molybdopterin) species.

X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Publishing Authors By Initials

GN George,CJ Doonan,RA Rothery,N Boroumand,JH Weiner,

For similar natural sciences: physics: radiation: radiation, ionizing: x-rays research abstracts see: natural sciences: physics: radiation: radiation, ionizing: x-rays research

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X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Inorganic chemistry

VOLUME: 46

Page Numbers: 2-4

Journal Abbreviation:

ISSN: 0020-1669

DAY: 8

MONTH: Jan

YEAR: 2007

X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 366543

X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Keywords Mesh Terms:

KEYWORDS: X-Rays

MESH TERMS: methods

Chemical & Substance for Abstract: X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase. Information

Substance Name: dimethyl sulfoxide reductase

Registry Number: EC 1.8.99.-

Grant and Affiliation Information for X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase.

AFFILIATION: Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada. g.george@usask.ca

Country: United States

AGENCY: United States NIGMS

GRANT: GM57375

ACRONYM: GM

MEDLINETA: Inorg Chem

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