[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling]

[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling] Research Abstract Details 

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[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling] Abstract Text:

Zhi-Min Zhang,Hui-Juan Wang,Zhong-Lai Liu,Guo-Mei Xiong,

Using our lab's improved protocol for faecal DNA extraction, the entire 753-bp DNA coding sequence of the nuclear brain-derived neurotrophic factor (BDNF) gene was cloned for the first time from Asiatic Black Bear Selenarctos thibetanus faecal samples with primers based on the reported sequence of the Malayan Bear BDNF gene. Hair was used as a positive control and the experiments were repeated several times to obtain reliable and identical results. Sequence analysis showed that the BDNF gene of Asiatic Black Bear was highly conserved compared to those of human and giant panda, with an identity of 94.5% and 98.9%, respectively. The deduced amino acid sequence of the mature protein was found to be identical to those of all the reported mammalians. According to gene sequence alignment, the giant panda appeared to be phylogenetically closer to Asiatic Black Bear than the lesser panda. This study represents the first time that a non-invasive method such as faecal sampling was used to analyze a functional nuclear BDNF gene of Asiatic Black Bear. It will not only provide important reference for the conservation and breeding of Asiatic Black Bear and open up new avenues of non-invasive sampling in the study of endangered wildlife, but also provide another molecular evidence for the study of relationship of Asiatic Black Bear and its related species.

[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling] Publishing Authors By Initials

ZM Zhang,HJ Wang,ZL Liu,GM Xiong,

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[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Yi chuan = Hereditas / Zhongguo yi chuan xue hui b

VOLUME: 28

Page Numbers: 665-71

Journal Abbreviation: Yi Chuan

ISSN: 0253-9772

DAY: 4

MONTH: Jun

YEAR: 2006

[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling] Information

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LANGUAGE: chi

NlmUniqueID: 9436478

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AFFILIATION: Genetics Laboratory, College of Life Sciences, Central China Normal University, Wuhan 430079, China. zzm_zhangzhimin@126.com

Country: China

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MEDLINETA: Yi Chuan

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