Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis.

Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Research Abstract Details 

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Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Abstract Text:

T E Radomisli,D C Moore,H J Barrach,H S Keeping,M G Ehrlich,

This study was performed to investigate the effect of loading on the biology of newly forming bone during limb lengthening. Unilateral 2.0 mm femoral lengthenings were performed in 20 male Sprague Dawley rats. Half (n = 10) of the animals were allowed to bear weight freely, while the other half were prevented from weight-bearing via an ipsilateral through-knee amputation. The animals in each group were sacrificed after one (n = 5) or four (n = 5) days of consolidation (post-operative days seven and 10, respectively). In situ hybridization for osteocalcin and collagen I, and antibody staining for collagen II and BMP 2/4 were used to evaluate the molecular influence of loading. There was more new bone in the distraction gap of the weight-bearing animals than there was in the non-weight-bearing animals. BMP 2/4 expression, and the messages for collagen I and osteocalcin, were more abundant in tissue from the weight-bearing animals; collagen II was higher in the non-weight-bearing animals. This suggests that early regenerate tissue is capable of responding to loading, and that weight-bearing appears to stimulate intramembranous ossification. These findings support the concept of early weight-bearing after limb lengthening.

Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Publishing Authors By Initials

TE Radomisli,DC Moore,HJ Barrach,HS Keeping,MG Ehrlich,

For similar biomechanics: weight-bearing research abstracts see: biomechanics: weight-bearing research

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Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of orthopaedic research : official publica

VOLUME: 19

Page Numbers: 1049-56

Journal Abbreviation: J. Orthop. Res.

ISSN: 0736-0266

DAY: 19

MONTH: Nov

YEAR: 2001

Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Information

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LANGUAGE: eng

NlmUniqueID: 8404726

Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Keywords Mesh Terms:

KEYWORDS: Weight-Bearing

MESH TERMS: analysis

Chemical & Substance for Abstract: Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis. Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for Weight-bearing alters the expression of collagen types I and II, BMP 2/4 and osteocalcin in the early stages of distraction osteogenesis.

AFFILIATION: Department of Orthopaedics, Brown University School of Medicine and Rhode Island Hospital, Providence 02903, USA.

Country: United States

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MEDLINETA: J Orthop Res

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