Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice.

Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Research Abstract Details 

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Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Abstract Text:

Yasuyuki Amoh,Michael Bouvet,Lingna Li,Kazuhiko Tsuji,A R Moossa,Kensei Katsuoka,Robert M Hoffman,

Nestin regulatory-element-driven green fluorescent protein (ND-GFP) transgenic mice highly express GFP in proliferating endothelial cells and nascent blood vessels. In the present study, we visualized angiogenesis in experimental lung and liver metastases by GFP imaging in the ND-GFP transgenic mice. The murine melanoma cell line, B16F10 expressing red fluorescent protein (RFP), was injected i.v. in ND-GFP mice. ND-GFP was highly expressed in proliferating nascent blood vessels in the tumors that developed in the lung after tail vein injection, and in the tumors that developed in the liver after portal vein injection of RFP-expressing melanoma cells. Liver metastasis and angiogenesis were imaged intravitally. Doxorubicin significantly decreased metastatic angiogenesis in the liver. These results demonstrate a new imageable model of angiogenesis in metastasis in the liver and the lung. This new model should enable further understanding of the onset of angiogenesis in metastasis and its effect on metastatic growth. The model will serve as a unique screen for inhibitors of angiogenesis of metastatic tumors. The fact that liver-metastasis angiogenesis can be imaged in the live animal enables real-time studies of the effect of angiogenesis inhibitors.

Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Publishing Authors By Initials

Y Amoh,M Bouvet,L Li,K Tsuji,AR Moossa,K Katsuoka,RM Hoffman,

For similar proteins: nerve tissue proteins research abstracts see: proteins: nerve tissue proteins research

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Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Clinical & experimental metastasis

VOLUME: 23

Page Numbers: 315-22

Journal Abbreviation: Clin. Exp. Metastasis

ISSN: 0262-0898

DAY: 30

MONTH: 11

YEAR: 2006

Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Information

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LANGUAGE: eng

NlmUniqueID: 8409970

Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Keywords Mesh Terms:

KEYWORDS: Nerve Tissue Proteins

MESH TERMS: diagnosis

Chemical & Substance for Abstract: Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Information

Substance Name: Doxorubicin

Registry Number: 23214-92-8

Grant and Affiliation Information for Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice.

AFFILIATION: AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: R21 CA109949-01

ACRONYM: CA

MEDLINETA: Clin Exp Metastasis

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