Visualisation of a kinesin-13 motor on microtubule end mimics.

Visualisation of a kinesin-13 motor on microtubule end mimics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Visualisation of a kinesin-13 motor on microtubule end mimics. Abstract Text:

An expanding collection of proteins localises to microtubule ends to regulate cytoskeletal dynamics and architecture by unknown molecular mechanisms. Electron microscopy is invaluable for studying microtubule structure, but because microtubule ends are heterogeneous, their structures are difficult to determine. We therefore investigated whether tubulin oligomers induced by the drug dolastatin could mimic microtubule ends. The microtubule end-dependent ATPase of kinesin-13 motors is coupled to microtubule depolymerisation. Significantly, kinesin-13 motor ATPase activity is stimulated by dolastatin-tubulin oligomers, suggesting, first, that these oligomers share properties with microtubule ends and, second, that the physical presence of an end is less important than terminal tubulin flexibility for microtubule end recognition by the kinesin-13 motor. Using electron microscopy, we visualised the kinesin-13 motor-dolastatin-tubulin oligomer interaction in nucleotide states mimicking steps in the ATPase cycle. This enabled us to detect conformational changes that the motor undergoes during depolymerisation. Our data suggest that such tubulin oligomers can be used to examine other microtubule end-binding proteins.

Visualisation of a kinesin-13 motor on microtubule end mimics. Publishing Authors By Initials

For similar macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research abstracts see: macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research

PUBMED ID PMID:

MEDLINE DATE:

Visualisation of a kinesin-13 motor on microtubule end mimics. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular biology

VOLUME: 377

Page Numbers: 647-54

Journal Abbreviation: J. Mol. Biol.

ISSN: 1089-8638

DAY: 4

MONTH: 02

YEAR: 2008

Visualisation of a kinesin-13 motor on microtubule end mimics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Visualisation of a kinesin-13 motor on microtubule end mimics. Keywords Mesh Terms:

KEYWORDS: Tubulin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Visualisation of a kinesin-13 motor on microtubule end mimics. Information

Substance Name: Kinesin

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Visualisation of a kinesin-13 motor on microtubule end mimics.

AFFILIATION: School of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK. c.moores@mail.cryst.bbk.ac.uk

Country: England

AGENCY: United States NCRR

GRANT: RR17573

ACRONYM: RR

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Visualisation of a kinesin-13 motor on microtubule end mimics Related Publications

• A simple approach to test for interaction between intervention and an individual-level variable in community randomized trials.
• An instrument to level tissue in paraffin embedding.
• Analysis of the weak interactions of ADP-Unc104 and ADP-kinesin with microtubules and their inhibition by MAP2c.
• Antibodies that identify only the active conformation of Gi family G protein {alpha} subunits.
• Antibody-mediated protection against genital herpes simplex virus type 2 disease in mice by Fc gamma receptor-dependent and -independent mechanisms.
• Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival.
• CXCR2 chemokine receptor antagonism enhances DOP opioid receptor function via allosteric regulation of the CXCR2-DOP receptor heterodimer.
• Cannabinoids for the treatment of neuropathic pain: Clinical evidence.
• Chemical methods in the evaluation of human contamination.
• Constitutive Activity of the Cannabinoid CB1 Receptor Regulates the Function of Co-expressed Mu Opioid Receptors.
• Early resolution of herpes simplex virus type 2 infection of the murine genital tract involves stimulation of genital parenchymal cells by gamma interferon.
• Epizootiology of trypanosomiases: some aspects of mathematical modelling.
• Exogenous Hsc70, but not thermal preconditioning, confers protection to motoneurons subjected to oxidative stress.
• G protein coupling and ligand selectivity of the D2L and D3 dopamine receptors.
• G-protein-coupled receptors: an update.
• In vitro methods to prepare astrocyte and motoneuron cultures for the investigation of potential in vivo interactions.
• Lucky 13-microtubule depolymerisation by kinesin-13 motors.
• Mallory-Weiss syndrome with esophageal obstruction secondary to adherent intraluminal thrombus.
• Maternal employment, breastfeeding, and health: Evidence from maternity leave mandates.
• Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta(2)-adrenoceptor and the ERK1/2 MAPKs: implications for beta(2)-adrenoceptor signalling via the ERK1/2 MAPKs.
• New aspects of g-protein-coupled receptor signalling and regulation.
• Reactivity of DNA guanyl radicals with phenolate anions.
• Regulation of heat shock protein 70 release in astrocytes: role of signaling kinases.
• Some determinants of attrition in prospective studies on aging.
• The patient's point of view.
• Treatment of psychoneurosis; modified CO2 abreactive technique.
• Urology in a modern health centre.
• Visualisation of a kinesin-13 motor on microtubule end mimics.
• Warburg (HARD +/- E) syndrome without retinal dysplasia: case report and review.
• c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons.