Virus inactivation by protein denaturants used in affinity chromatography.

Virus inactivation by protein denaturants used in affinity chromatography. Research Abstract Details 

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Virus inactivation by protein denaturants used in affinity chromatography. Abstract Text:

Peter L Roberts,David Lloyd,Peter L Roberts,David Lloyd,

Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by 0.5 M sodium hydroxide, 6 M guanidinium thiocyanate, 8 M urea and 70% ethanol. However, pH 2.6, 3 M sodium thiocyanate, 6 M guanidinium chloride and 20% ethanol, while effectively inactivating the enveloped viruses, did not inactivate polio-1. These studies demonstrate that protein denaturants are generally effective for virus inactivation but with the limitation that only some may inactivate non-enveloped viruses. The use of protein denaturants, together with virus reduction steps in the manufacturing process should ensure that viral cross contamination between manufacturing batches of therapeutic biological products is prevented and the safety of the product ensured.

Virus inactivation by protein denaturants used in affinity chromatography. Publishing Authors By Initials

PL Roberts,D Lloyd,PL Roberts,D Lloyd,

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Virus inactivation by protein denaturants used in affinity chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biologicals : journal of the International Associa

VOLUME: 35

Page Numbers: 343-7

Journal Abbreviation: Biologicals

ISSN: 1045-1056

DAY: 24

MONTH: 05

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Bio Products Laboratory, Dagger Lane, Elstree, Hertfordshire, UK. peter.roberts@bpl.co.uk

Country: England

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MEDLINETA: Biologicals

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