Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity.

Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Research Abstract Details 

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Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Abstract Text:

Zhiping Cao,Lijuan Liu,Marvin Lickey,Aundrea Graves,Tony Pham,Barbara Gordon,Zhiping Cao,Lijuan Liu,Marvin Lickey,Aundrea Graves,Tony Pham,Barbara Gordon,

NMDA receptors (NMDARs) are important in developmental plasticity in the visual cortex. The NR2A and NR2B subunits of this receptor develop with different time courses, suggesting that they play different roles in plasticity. To understand the role of the NR2B subunit, we knocked-down NR2B gene expression in visual cortex by injecting a recombinant adenovirus containing an antisense NR2B oligonucleotide. To assess knock-down, we injected the recombinant adenovirus into the right visual cortex of rats (p22) or mice (p30). Eight days later we perfused the animals and processed the visual cortex for NMDAR subunit immunoreactivity (IR). NR2B-IR was depleted dramatically in the neuropil near the injection. Depletion was more modest in the neuronal somata. Surprisingly, NR2A-IR was also reduced, but NR1-IR was not reduced. To assess the functional effects of depletion, we measured ocular dominance plasticity with monocular deprivation (MD). We compared mice receiving the NR2B antisense virus with mice receiving virus containing only the GFP sequence and mice receiving no injection. All injections were between p26 and p29 in the right cortex and bilateral recordings were performed 6-8 days later. Animals receiving the antisense virus lost plasticity if the right eye was deprived. If the left eye was deprived, the cortex was normally plastic bilaterally. Injection of control virus had no effect on plasticity. The data indicate that ocular dominance plasticity requires normal NMDARs in the hemisphere ipsilateral to the deprived eye but not in the hemisphere contralateral to the deprived eye.

Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Publishing Authors By Initials

Z Cao,L Liu,M Lickey,A Graves,T Pham,B Gordon,Z Cao,L Liu,M Lickey,A Graves,T Pham,B Gordon,

For similar nervous system: central nervous system: brain: prosencephalon: telencephalon: cerebrum: cerebral cortex: occipital lobe: visual cortex research abstracts see: nervous system: central nervous system: brain: prosencephalon: telencephalon: cerebrum: cerebral cortex: occipital lobe: visual cortex research

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Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental brain research. Experimentelle Hirnf

VOLUME: 177

Page Numbers: 64-77

Journal Abbreviation:

ISSN: 0014-4819

DAY: 30

MONTH: 08

YEAR: 2006

Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 43312

Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Keywords Mesh Terms:

KEYWORDS: Visual Cortex

MESH TERMS: physiology

Chemical & Substance for Abstract: Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Virally mediated knock-down of NR2 subunits ipsilateral to the deprived eye blocks ocular dominance plasticity.

AFFILIATION: Portland VA Medical Center, PO Box 1034/P3ANES, Portland, OR 97239, USA.

Country: Germany

AGENCY: United States NEI

GRANT: R01 EY014238

ACRONYM: EY

MEDLINETA: Exp Brain Res

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