Viral DNA from cells infected with porcine circovirus.

Viral DNA from cells infected with porcine circovirus. Research Abstract Details 

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Viral DNA from cells infected with porcine circovirus. Abstract Text:

I Tischer,H J Buhk,

It has been demonstrated earlier that the genome of the porcine circovirus (PCV) isolated from virions is a circular single-stranded DNA (ssDNA). This report describes the isolation and characterization of PCV-specific DNA from infected cell cultures. Two types of double-stranded DNA (dsDNA) were observed which behaved like supercoiled and relaxed circular molecules, respectively. Both types of dsDNA displayed infectivity in transfection experiments and are regarded as replicative forms (RF) of the viral genome. In addition to complete single-stranded viral genomes and RF DNA molecules, PCV-specific subgenomic fractions of DNA molecules with a sedimentation coefficient of about 5 S were isolated from infected tissue culture cells. This 5 S DNA was also isolated from purified virions. It is single-stranded and represents a defined region of the viral genome.

Viral DNA from cells infected with porcine circovirus. Publishing Authors By Initials

I Tischer,HJ Buhk,

For similar viruses: virion research abstracts see: viruses: virion research

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Viral DNA from cells infected with porcine circovirus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Bakteriologie, Mikrobiologie, und

VOLUME: 270

Page Numbers: 280-7

Journal Abbreviation:

ISSN: 0176-6724

DAY: 12

MONTH: Nov

YEAR: 1988

Viral DNA from cells infected with porcine circovirus. Information

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LANGUAGE: eng

NlmUniqueID: 8403032

Viral DNA from cells infected with porcine circovirus. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: genetics

Chemical & Substance for Abstract: Viral DNA from cells infected with porcine circovirus. Information

Substance Name: Aspergillus Nuclease S1

Registry Number: EC 3.1.30.1

Grant and Affiliation Information for Viral DNA from cells infected with porcine circovirus.

AFFILIATION: Robert Koch-Institut, Bundesgesundheitamt, Berlin.

Country: GERMANY, WEST

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MEDLINETA: Zentralbl Bakteriol Mikrobiol

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