Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos.

Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Research Abstract Details 

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Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Abstract Text:

Elayne Provost,Jerry Rhee,Steven D Leach,Elayne Provost,Jerry Rhee,Steven D Leach,

We have adapted a novel multicistronic gene expression system involving viral peptides to the zebrafish. The viral 2A peptide allows production of multiple protein products from a single transgene. Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA's allows the stoichiometric translation of multiple unfused protein products. To test this system in zebrafish, we generated two different tandem reporter constructs employing eGFP and mCherry, separated by 2A peptide sequence. Using this system, we produced transgenic zebrafish in which fluorophores were produced as independent proteins from a single transcript. The successful application of this technology in zebrafish will be valuable for visually marking transgenic embryos and transgene-expressing cells, or in any situation where reliable expression of multiple transgenes is desired.

Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Publishing Authors By Initials

E Provost,J Rhee,SD Leach,E Provost,J Rhee,SD Leach,

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Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genesis (New York, N.Y. : 2000)

VOLUME: 45

Page Numbers: 625-9

Journal Abbreviation: Genesis

ISSN: 1526-954X

DAY: 22

MONTH: Oct

YEAR: 2007

Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Information

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LANGUAGE: eng

NlmUniqueID: 100931242

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Grant and Affiliation Information for Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos.

AFFILIATION: Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: F32-DK077480

ACRONYM: DK

MEDLINETA: Genesis

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