Vinculin controls focal adhesion formation by direct interactions with talin and actin.

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Abstract Text:

Jonathan D Humphries,Pengbo Wang,Charles Streuli,Benny Geiger,Martin J Humphries,Christoph Ballestrem,Jonathan D Humphries,Pengbo Wang,Charles Streuli,Benny Geiger,Martin J Humphries,Christoph Ballestrem,

Focal adhesions (FAs) regulate cell migration. Vinculin, with its many potential binding partners, can interconnect signals in FAs. Despite the well-characterized structure of vinculin, the molecular mechanisms underlying its action have remained unclear. Here, using vinculin mutants, we separate the vinculin head and tail regions into distinct functional domains. We show that the vinculin head regulates integrin dynamics and clustering and the tail regulates the link to the mechanotransduction force machinery. The expression of vinculin constructs with unmasked binding sites in the head and tail regions induces dramatic FA growth, which is mediated by their direct interaction with talin. This interaction leads to clustering of activated integrin and an increase in integrin residency time in FAs. Surprisingly, paxillin recruitment, induced by active vinculin constructs, occurs independently of its potential binding site in the vinculin tail. The vinculin tail, however, is responsible for the functional link of FAs to the actin cytoskeleton. We propose a new model that explains how vinculin orchestrates FAs.

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Publishing Authors By Initials

JD Humphries,P Wang,C Streuli,B Geiger,MJ Humphries,C Ballestrem,JD Humphries,P Wang,C Streuli,B Geiger,MJ Humphries,C Ballestrem,

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

PUBMED ID PMID:

MEDLINE DATE:

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 179

Page Numbers: 1043-57

Journal Abbreviation: J. Cell Biol.

ISSN: 1540-8140

DAY: 3

MONTH: Dec

YEAR: 2007

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Vinculin controls focal adhesion formation by direct interactions with talin and actin. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Vinculin controls focal adhesion formation by direct interactions with talin and actin. Information

Substance Name: Actomyosin

Registry Number: 9013-26-7

Grant and Affiliation Information for Vinculin controls focal adhesion formation by direct interactions with talin and actin.

AFFILIATION: Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester M13 9PT, England, UK.

Country: United States

AGENCY: United Kingdom Wellcome T

GRANT: 077100

ACRONYM:

MEDLINETA: J Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Vinculin controls focal adhesion formation by direct interactions with talin and actin Related Publications

• Vinculin controls focal adhesion formation by direct interactions with talin and actin.