Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation.

Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Research Abstract Details 

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Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Abstract Text:

B H Zinselmeyer,J N Lynch,X Zhang,T Aoshi,M J Miller,

Leukocyte recruitment is a key host defense mechanism to infection and a salient feature of autoimmune diseases such as arthritis. The cell dynamics of these processes are difficult to study due to the challenge of tracking cells flowing in the circulation and migrating through light scattering tissues. Here, we describe a noninvasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. In the absence of inflammation, cells moved > several hundred mum/s in vessels and only rarely adhered to endothelium or entered the tissue parenchyma. In response to bacterial infection, neutrophils moved in small capillaries at reduced speeds of (14-45mum/min) and rolled in larger vessels at 5-60 mum/min. Within minutes of adoptive transfer, neutrophils entered the connective tissue and crawled with a median velocity of 7.3 mum/min. 2P imaging has excellent spatiotemporal resolution and is a promising in vivo approach to study the cellular basis of inflammation.

Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Publishing Authors By Initials

BH Zinselmeyer,JN Lynch,X Zhang,T Aoshi,MJ Miller,

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Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Inflammation research : official journal of the Eu

VOLUME: 57

Page Numbers: 93-6

Journal Abbreviation: Inflamm. Res.

ISSN: 1023-3830

DAY: 28

MONTH: Mar

YEAR: 2008

Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Video-rate two-photon imaging of mouse footpad - a promising model for studying leukocyte recruitment dynamics during inflammation.

AFFILIATION: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.

Country: Switzerland

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MEDLINETA: Inflamm Res

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