Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase.

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Abstract Text:

R Prisca ,John E Cronan,R Prisca ,John E Cronan,R Prisca Massengo-Tiassé,John E Cronan,

Enoyl-acyl carrier protein (ACP) reductase catalyzes the last step of the fatty acid elongation cycle. The paradigm enoyl-ACP reductase is the FabI protein of Escherichia coli that is the target of the antibacterial compound, triclosan. However, some Gram-positive bacteria are naturally resistant to triclosan due to the presence of the triclosan-resistant enoyl-ACP reductase isoforms, FabK and FabL. The genome of the Gram-negative bacterium, Vibrio cholerae lacks a gene encoding a homologue of any of the three known enoyl-ACP reductase isozymes suggesting that this organism encodes a novel fourth enoyl-ACP reductase isoform. We report that this is the case. The gene encoding the new isoform, called FabV, was isolated by complementation of a conditionally lethal E. coli fabI mutant strain and was shown to restore fatty acid synthesis to the mutant strain both in vivo and in vitro. Like FabI and FabL, FabV is a member of the short chain dehydrogenase reductase superfamily, although it is considerably larger (402 residues) than either FabI (262 residues) or FabL (250 residues). The FabV, FabI and FabL sequences can be aligned, but only poorly. Alignment requires many gaps and yields only 15% identical residues. Thus, FabV defines a new class of enoyl-ACP reductase. The native FabV protein has been purified to homogeneity and is active with both crotonyl-ACP and the model substrate, crotonyl-CoA. In contrast to FabI and FabL, FabV shows a very strong preference for NADH over NADPH. Expression of FabV in E. coli results in markedly increased resistance to triclosan and the purified enzyme is much more resistant to triclosan than is E. coli FabI.

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Publishing Authors By Initials

RP ,JE Cronan,RP ,JE Cronan,RP Massengo-Tiassé,JE Cronan,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 1308-16

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 21

MONTH: 11

YEAR: 2007

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase.

AFFILIATION: Departments of Microbiology and Biochemistry, University of Illinois, Urbana, Illinois 61801.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase Related Publications

• Acyl carrier protein phosphodiesterase (AcpH) of Escherichia coli is a non-canonical member of the HD phosphatase/phosphodiesterase family.
• CT appearance of splenic injuries managed nonoperatively.
• Coordinate expression of the acetyl coenzyme A carboxylase genes, accB and accC, is necessary for normal regulation of biotin synthesis in Escherichia coli.
• Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.
• Fourteen percent mortality rate in catheter-drainage series: setting the record straight.
• Genetic Interaction Between the Escherichia coli AcpT Phosphopantetheinyl Transferase and the YejM Inner Membrane Protein.
• Imaging-guided percutaneous radiofrequency ablation of solid renal masses: techniques and outcomes of 38 treatment sessions in 32 consecutive patients.
• In vivo functional analyses of the type II acyl carrier proteins of fatty acid biosynthesis.
• In vivo resolution of conflicting in vitro results: synthesis of biotin from dethiobiotin does not require pyridoxal phosphate.
• My first job: the transition from residency to employment-what the employer and employee should know.
• Radiology of male periurethral structures.
• Referrals to a dedicated cardiac MRI service: who sends what?
• The Lactococcus lactis FabF fatty acid synthetic enzyme can functionally replace both the FabB and FabF proteins of Escherichia coli and the FabH protein of Lactococcus lactis.
• Thyroid nodules: is it time to turn off the US machines?
• Understanding ethnic disparities in the use of total joint arthroplasty: Application of the health belief model.
• Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase.