Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Abstract Text:

M Takagi,T Otoi,A Boediono,S Saha,T Suzuki,

Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1.6 M propylene glycol (PG), 1.8 M ethylene glycol (EG), 1.1 M diethylene glycol (DEG) or 1.3 M ethylene glycol monomethyl ether (EME) for 10 to 20 min in modified phosphate buffered saline. (mPBS) supplemented with 10% superovulated cow serum. The embryos were loaded into 0.25-ml plastic straws and were placed directly into a 0 degrees C alcohol bath chamber and held for 2 min. They were cooled from 0 degrees C to -5.5 degrees C at 1 degrees C/min and then seeded, followed by a 10-min holding period at -5.5 degrees C. The straws were then cooled to -30 degrees C at 0.3 degrees C/min before plunging into liquid nitrogen. Embryos were thawed and placed directly into the culture medium and washed 3 times. The survival rates of the Day-9 embryos based on reappearance of blastocoele, expansion, and hatching after 48 h of post-thaw culture were significantly lower (P<0.01) than those of the Day-7 and 8 embryos, in all of the cryoprotectants tested. On the other hand, while the reappearance of blastocoele and expansion of blastocysts after 48 h of post-thaw culture were not significantly different among each cryoprotectant, the percentage of hatching blastocysts were significantly different between DEG and EME (P<0.05), between DEG and EG (P<0.01) and between PG and EG (P<0.05). These findings demonstrate that the age of the embryo (Day 7 and 8) is very important for the successful freezing of IVF bovine embryos. Also, as to the hatching rates, EME and EG are superior as cryoprotectants than the other 2 cryoprotectants tested.

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Publishing Authors By Initials

M Takagi,T Otoi,A Boediono,S Saha,T Suzuki,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 41

Page Numbers: 915-21

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: 05

YEAR: 1994

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 421510

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.

AFFILIATION: United Graduate School of Veterinary Science Yamaguchi University, Yamaguchi 753, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Theriogenology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants Related Publications

• A uniformly cleaved epitaxically grown diamond crystal for synchrotron radiation.
• Absolute soft X-ray measurements using an ion chamber.
• Agmatine suppresses mesangial cell proliferation by modulating polyamine metabolism.
• Attention induces synchronization-based response gain in steady-state visual evoked potentials.
• Behavior of Medaka fish under distributed gravity.
• Branched-chain amino acid supplementation during the endoscopic injection sclerotherapy for esophageal varices may be useful for maintenance of nutritional status in patients with liver cirrhosis.
• Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center.
• Cytokine production of CD8+ immune T cells but not of CD4+ T cells from Toxoplasma gondii-infected mice is polarized to a type 1 response following stimulation with tachyzoite-infected macrophages.
• Developmental capacity of bovine oocytes cryopreserved after maturation in vitro and of frozen-thawed bovine embryos derived from frozen mature oocytes.
• Developmental capacity of bovine oocytes frozen in different cryoprotectants.
• Effects of different lots of semen from the same bull on in vitro development of bovine oocytes fertilized in vitro.
• Effects of pressure on the phase transition of bilayers in liposomes. Influence of cholesterol and alpha-tocopherol.
• Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line.
• Expression of bHLH Transcription Factors and IGFs in the Non-sensory Patches, Olfactory Epithelium and Vomeronasal Organ.
• Fasting plasma ghrelin levels reflect malnutrition state in patients with liver cirrhosis.
• Hepatocyte-specific Pten-deficient mice as a novel model for nonalcoholic steatohepatitis and hepatocellular carcinoma.
• Hyperplasia and carcinomas in Pten-deficient mice and reduced PTEN protein in human bladder cancer patients.
• Improvement in cognitive function after radical excision of an anterior skull base meningioma - a report of 2 cases.
• Independent representation of parts and the relations between them: evidence from integrative agnosia.
• Prognosis-predicting equations using histological scores contribute to the choice of steroid therapy in IgA nephropathy: Multivariate analysis on 275 adult patients.
• Proteome analysis of glial cells treated by radiation or hydroperoxide.
• Relationship between progesterone and estradiol contents of follicular fluid and the morphological appearance of granulosa cells of follicles coexisting with corpora lutea in bovine ovaries.
• Safety of ablation of the sigmoid and transverse sinuses: an experimental study.
• Species specific differences in the ratio of short to long loop nephrons in the kidneys of laboratory rodents.
• Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone.
• Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713.
• The A-site finger in 23 S rRNA acts as a functional attenuator for translocation.
• Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.
• [High performance liquid chromatographic determination of reducing sugars in fruit juices with laser resonance Raman detection]
• [Selective extraction of organotin from biological sample by supercritical fluid extraction]