Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA.

Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA. Research Abstract Details 

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Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA. Abstract Text:

Jennifer T Wang,Lauren E McElvain,Sean P J Whelan,Jennifer T Wang,Lauren E McElvain,Sean P J Whelan,

Many viruses of eukaryotes that use mRNA cap-dependent translation strategies have evolved alternate mechanisms to generate the mRNA cap compared to their hosts. The most divergent of these mechanisms are those used by nonsegmented negative-sense (NNS) RNA viruses, which evolved a capping enzyme that transfers RNA onto GDP, rather than GMP onto the 5' end of the RNA. Working with vesicular stomatitis virus (VSV), a prototype of the NNS RNA viruses, we show that mRNA cap formation is further distinct, requiring a specific cis-acting signal in the RNA. Using recombinant VSV, we determined the function of the eight conserved positions of the gene-start sequence in mRNA initiation and cap formation. Alterations to this sequence compromised mRNA initiation and separately formation of the GpppA cap structure. These studies provide genetic and biochemical evidence that the mRNA capping apparatus of VSV evolved an RNA capping machinery that functions in a sequence-specific manner.

Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA. Publishing Authors By Initials

JT Wang,LE McElvain,SP Whelan,JT Wang,LE McElvain,SP Whelan,

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Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 81

Page Numbers: 11499-506

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 8

MONTH: 08

YEAR: 2007

Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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Grant and Affiliation Information for Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA.

AFFILIATION: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI059371-03

ACRONYM: AI

MEDLINETA: J Virol

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