Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming.

Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Research Abstract Details 

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Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Abstract Text:

Wai-ki Yiu,Stephen W K Cheng,Bauer E Sumpio,

PURPOSE: The underlying mechanisms for the reduction in restenosis caused by cryoplasty for peripheral atherosclerotic lesions are not well understood. Because vascular smooth muscle cells (SMCs) are known to play a critical role in restenosis and neointimal hyperplasia, the aim of this study was to determine SMC survival under conditions of "supercooling" and/or rewarming. MATERIALS AND METHODS: Bovine aortic SMCs were supercooled to -10 degrees C for 0, 60, or 120 seconds with a custom-designed conduction cooling stage and then rewarmed to 37 degrees C in an incubator for 0, 12, or 24 hours. A terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay was used to measure the degree of apoptosis. Activation of Akt (ie, protein kinase B), a key signal protein involved in cell survival, was assessed by Western blot analysis. RESULTS: An increase in apoptotic SMCs was observed with increasing supercooling and rewarming time. Akt was significantly activated at only the most severe condition (120 seconds of supercooling and 24 hours of rewarming), which showed a 2.03-fold increase compared with the group without rewarming. CONCLUSIONS: The data suggest that SMC apoptosis occurs with supercooling and rewarming. Protective cell survival mechanisms were activated only late in the rewarming phase. This may partially explain the long-term patency observed with cryoplasty of atherosclerotic peripheral lesions.

Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Publishing Authors By Initials

WK Yiu,SW Cheng,BE Sumpio,

For similar therapeutics: rewarming research abstracts see: therapeutics: rewarming research

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Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of vascular and interventional radiology :

VOLUME: 17

Page Numbers: 1971-7

Journal Abbreviation:

ISSN: 1051-0443

DAY: 3

MONTH: Dec

YEAR: 2006

Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Information

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LANGUAGE: eng

NlmUniqueID: 9203369

Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming. Keywords Mesh Terms:

KEYWORDS: Rewarming

MESH TERMS: cytology

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Grant and Affiliation Information for Vascular smooth muscle cell apoptosis induced by "supercooling" and rewarming.

AFFILIATION: Department of Vascular Surgery, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520-8062, USA, and Department of Vascular Surgery, Queen Mary Hospital, Hong Kong.

Country: United States

AGENCY: United States NHLBI

GRANT: R01-HL47345

ACRONYM: HL

MEDLINETA: J Vasc Interv Radiol

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