Vascular metabolic dysfunction and lipotoxicity.

Vascular metabolic dysfunction and lipotoxicity. Research Abstract Details 

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Vascular metabolic dysfunction and lipotoxicity. Abstract Text:

H M Mattern,C D Hardin,H M Mattern,C D Hardin,

The purpose of this study was to determine the role of lipotoxicity in vascular smooth muscle (VSM). C(1)-BODIPY 500/510 C(12) used to assess the ability of VSM A7r5 cells to transport long-chain fatty acids showed that lipid transport did not appear to limit metabolism. Thin layer chromatography revealed that storage of transported fatty acid occurred primarily as mono- and diglycerides and fatty acids but not as triglycerides. We used lipid-induced apoptosis as a measure of lipotoxicity and found that 1.5 mM palmitate (6.8:1) bound to albumin resulted in a 15-fold increase in the number of apoptotic cells compared to the control at 24 hours. This apoptosis did not seem to be due to an increase in reactive oxygen species (ROS) since VSM cells incubated in palmitate showed less ROS production than cells incubated in albumin only. Similar exposure to oleate did not significantly increase the number of apoptotic cells compared to the control. Oleate actually significantly attenuated the apoptosis induced by palmitate, suggesting that unsaturated fatty acids have a protective effect on cells undergoing palmitate-induced apoptosis. These results suggest that vascular smooth muscle is vulnerable to lipotoxicity and that this lipotoxicity may play a role in the development of atherosclerosis.

Vascular metabolic dysfunction and lipotoxicity. Publishing Authors By Initials

HM Mattern,CD Hardin,HM Mattern,CD Hardin,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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MEDLINE DATE:

Vascular metabolic dysfunction and lipotoxicity. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Physiological research / Academia Scientiarum Bohe

VOLUME: 56

Page Numbers: 149-58

Journal Abbreviation:

ISSN: 0862-8408

DAY: 23

MONTH: 03

YEAR: 2006

Vascular metabolic dysfunction and lipotoxicity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9112413

Vascular metabolic dysfunction and lipotoxicity. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Vascular metabolic dysfunction and lipotoxicity. Information

Substance Name: Palmitic Acid

Registry Number: 57-10-3

Grant and Affiliation Information for Vascular metabolic dysfunction and lipotoxicity.

AFFILIATION: Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65212, USA.

Country: Czech Republic

AGENCY: United States NIDDK

GRANT: DK 60668

ACRONYM: DK

MEDLINETA: Physiol Res

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