Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts.

Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Research Abstract Details 

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Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Abstract Text:

U Mayr-Wohlfart,J Waltenberger,H Hausser,S Kessler,K-P ,C Dehio,W Puhl,R E Brenner,

Recent studies have indicated a critical role for vascular endothelial growth factor (VEGF) during the process of endochondral ossification, in particular in coupling cartilage resorption with bone formation. Therefore, we studied the chemoattractive and proliferative properties of human VEGF-A on primary human osteoblasts (PHO) and compared these data with the effects of human basic fibroblast growth factor (bFGF) and human bone morphogenetic protein-2 (BMP-2). Furthermore, initial experiments were carried out to characterize VEGF-binding proteins on osteoblastic cells possibly involved in the response. For the first time, to our knowledge, we could demonstrate a chemoattractive effect of VEGF-A, but not VEGF-E, on primary human osteoblasts. The effect of VEGF-A was dose-dependent and did not reach a maximum within the concentration range tested (up to 10 ng/mL). The maximal effect observed was a chemotactic index (CI) of 2 at a concentration of 10 ng/mL. bFGF and BMP-2 exhibited maxima at 1.0 ng/mL with CI values of 2.5 and 2, respectively. In addition to its effect on cell migration, VEGF-A stimulated cell proliferation by up to 70%. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed the expression of VEGF receptors VEGFR-1 (Flt-1), VEGFR-2 (Kdr), and VEGFR-3 (Flt-4), as well as neuropilin-1 and -2. An in vitro kinase assay failed to demonstrate activation of VEGFR-2 upon stimulation with either VEGF-E or VEGF-A, consistent with the idea that the effect of VEGF-A on primary human osteoblasts is mediated via VEGFR-1. Taken together, our data establish that human osteoblasts respond to VEGF-A, suggesting a functional role for this growth factor in bone formation and remodeling.

Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Publishing Authors By Initials

U Mayr-Wohlfart,J Waltenberger,H Hausser,S Kessler,KP ,C Dehio,W Puhl,RE Brenner,

For similar peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research abstracts see: peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research

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Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bone

VOLUME: 30

Page Numbers: 472-7

Journal Abbreviation: Bone

ISSN: 8756-3282

DAY: 19

MONTH: Mar

YEAR: 2002

Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Information

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LANGUAGE: eng

NlmUniqueID: 8504048

Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Keywords Mesh Terms:

KEYWORDS: Vascular Endothelial Growth Factor A

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts. Information

Substance Name: Receptors, Vascular Endothelial Growth F

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts.

AFFILIATION: Orthopaedic Department (RKU), University of Ulm, Ulm, Germany.

Country: United States

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MEDLINETA: Bone

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