Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway.

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Abstract Text:

Heidi J Zapata,Masako Nakatsugawa,Jennifer F Moffat,

The transcription factors ATF-2 and c-Jun are important for transactivation of varicella-zoster virus (VZV) genes. c-Jun is activated by the c-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase pathway that responds to stress and cytokines. To study the effects of VZV on this pathway, confluent human foreskin fibroblasts were infected with cell-associated VZV for 1 to 4 days. Immunoblots showed that phosphorylated JNK and c-Jun levels increased in VZV-infected cells, and kinase assays determined that phospho-JNK was active. Phospho-JNK was detected after 24 h, and levels rose steadily over 4 days in parallel with accumulation of VZV antigen. The two main activators of JNK are MKK4 and MKK7, and levels of their active, phosphorylated forms also increased. The competitive inhibitor of JNK, SP600125, caused a dose-dependent reduction in VZV yield (50% effective concentration, congruent with 8 microM). Specificity was verified by immunoblotting; phospho-c-Jun was eliminated by 18 microM SP600125 in VZV-infected cells. Immunofluorescent confocal microscopy showed that phospho-c-Jun and most of phospho-JNK were in the nuclei of VZV-infected cells; some phospho-JNK was in the cytoplasm. MKK4, MKK7, JNK, and phospho-JNK were detected by immunoblotting in purified preparations of VZV virions, but c-Jun was absent. JNK was located in the virion tegument, as determined by biochemical fractionation and immunogold transmission electron microscopy. Overall, these results demonstrate the importance of the JNK pathway for VZV replication and advance the idea that JNK is a useful drug target against VZV.

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Publishing Authors By Initials

HJ Zapata,M Nakatsugawa,JF Moffat,

For similar viruses: virion research abstracts see: viruses: virion research

PUBMED ID PMID:

MEDLINE DATE:

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 977-90

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 1

MONTH: 11

YEAR: 2006

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway. Information

Substance Name: JNK Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway.

AFFILIATION: Department of Microbiology and Immunology, State University of New York Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210, USA.

Country: United States

AGENCY: United States NIAID

GRANT: F31 AI061848

ACRONYM: AI

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway Related Publications

• A study of perioperative lumbar cerebrospinal fluid pressure in patients undergoing acoustic neuroma surgery.
• Are facial nerve outcomes worse following surgery for cystic vestibular schwannoma?
• Biophysical methods: structure, dynamics and gorgeous images.
• Change in hearing and tinnitus in conservatively managed vestibular schwannomas.
• Cloning freedom: criminalization or empowerment in reproductive policy?
• Composition and concentration of hydrocarbons in sediment samples from the oil producing area of the East Shetland Basin, Scotland.
• Crystal structure of the chromophore binding domain of an unusual bacteriophytochrome, RpBphP3, reveals residues that modulate photoconversion.
• Dangers of corticosteroids in phaeochromocytoma.
• Development and application of an analytical method for the determination of storage lipids, fatty acids and fatty alcohols in Calanus finmarchicus.
• Drosophila genetics for the analysis of neurobiological disease.
• Feasibility and Realization of Single-Pulse Laue Diffraction on Macromolecular Crystals at ESRF.
• Laue crystallography for studying rapid reactions.
• Management of gastrointestinal nematode parasites on sheep farms in New Zealand.
• Micromorphological investigations into root penetration in a landfill mineral cap, Hertfordshire, UK.
• Molecular aspects of toxicology by D. E. Hathway. Royal Society of Chemistry, London, 1984, pp. 304, ISBN 0-85186-068-0, pound27.50.
• N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa.
• Passive sampling: partition coefficients for a silicone rubber reference phase.
• Preliminary assessment of polybrominated diphenyl ethers (PBDEs) in the Scottish aquatic environment, including the Firth of Clyde.
• Preoperative audiovestibular handicap in patients with vestibular schwannoma.
• Providing help and support for caregivers.
• Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli.
• Quality of life in patients with cancer.
• Radiologic changes in the thymoma-hypogammaglobulinemia syndrome.
• Should we tag people with dementia?
• Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA.
• Structure of the redox sensor domain of Azotobacter vinelandii NifL at atomic resolution: signaling, dimerization, and mechanism.
• The clinical characteristics of tinnitus in patients with vestibular schwannoma.
• Time-resolved crystallographic studies of the heme domain of the oxygen sensor FixL: structural dynamics of ligand rebinding and their relation to signal transduction.
• Varicella-zoster virus infection of human fibroblast cells activates the c-Jun N-terminal kinase pathway.
• Varicella-zoster virus infection of human foreskin fibroblast cells results in atypical cyclin expression and cyclin-dependent kinase activity.