Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread.

Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread. Research Abstract Details 

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Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread. Abstract Text:

Yoshiaki Yamagishi,Tomohiko Sadaoka,Hironori Yoshii,Pranee Somboonthum,Takayoshi Imazawa,Kazuhiro Nagaike,Keiichi Ozono,Koichi Yamanishi,Yasuko Mori,Yoshiaki Yamagishi,Tomohiko Sadaoka,Hironori Yoshii,Pranee Somboonthum,Takayoshi Imazawa,Kazuhiro Nagaike,Keiichi Ozono,Koichi Yamanishi,Yasuko Mori,

Although envelope glycoprotein M (gM) is highly conserved among herpesviruses, the varicella-zoster virus (VZV) gM homolog has never been investigated. Here we characterized the VZV gM homolog and analyzed its function in VZV-infected cells. The VZV gM homolog was expressed on virions as a glycoprotein modified with a complex N-linked oligosaccharide and localized mainly to the Golgi apparatus and the trans-Golgi network in infected cells. To analyze its function, a gM deletion mutant was generated using the bacterial artificial chromosome system in Escherichia coli, and the virus was reconstituted in MRC-5 cells. VZV is highly cell associated, and infection proceeds mostly by cell-to-cell spread. Compared with wild-type VZV, the gM deletion mutant showed a 90% reduction in plaque size and 50% of the cell-to-cell spread in MRC-5 cells. The analysis of infected cells by electron microscopy revealed numerous aberrant vacuoles containing electron-dense materials in cells infected with the deletion mutant virus but not in those infected with wild-type virus. However, enveloped immature particles termed L particles were found at the same level on the surfaces of cells infected with either type of virus, indicating that envelopment without a capsid might not be impaired. These results showed that VZV gM is important for efficient cell-to-cell virus spread in cell culture, although it is not essential for virus growth.

Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread. Publishing Authors By Initials

Y Yamagishi,T Sadaoka,H Yoshii,P Somboonthum,T Imazawa,K Nagaike,K Ozono,K Yamanishi,Y Mori,Y Yamagishi,T Sadaoka,H Yoshii,P Somboonthum,T Imazawa,K Nagaike,K Ozono,K Yamanishi,Y Mori,

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Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 82

Page Numbers: 795-804

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 31

MONTH: 10

YEAR: 2007

Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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Grant and Affiliation Information for Varicella-zoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread.

AFFILIATION: Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.

Country: United States

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MEDLINETA: J Virol

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