Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images.

Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Research Abstract Details 

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Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Abstract Text:

John Virostko,Alvin C Powers,E Duco Jansen,

We characterize the capabilities and limitations of the Living Image Software 3D Analysis package (Xenogen, Alameda, California) in the reconstruction of calibrated light sources. Sources shallower than the mean free path of light propagation suffered reconstruction inaccuracy. For sources deeper than the mean free path, the average error in depth and intensity reconstruction was less than 4% and 12%, respectively, for homogeneous tissue. The reconstruction of luminescent beads implanted within an optically heterogeneous mouse abdomen proved less accurate. The ability to distinguish multiple sources decreased with increasing source depth. A number of factors influence the accuracy of light source reconstruction.

Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Publishing Authors By Initials

J Virostko,AC Powers,ED Jansen,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

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Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Applied optics

VOLUME: 46

Page Numbers: 2540-7

Journal Abbreviation:

ISSN: 0003-6935

DAY: 1

MONTH: May

YEAR: 2007

Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Information

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LANGUAGE: eng

NlmUniqueID: 247660

Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images. Information

Substance Name: Luminescent Proteins

Registry Number: 0

Grant and Affiliation Information for Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images.

AFFILIATION: Department of Biomedical Engineering, VU Station B No. 351631, Vanderbilt University, Nashville, Tennessee 37235, USA. jack.virostko@vanderbilt.edu

Country: United States

AGENCY: United States NIDDK

GRANT: DK69603

ACRONYM: DK

MEDLINETA: Appl Opt

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