Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples.

Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Research Abstract Details 

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Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Abstract Text:

N ,A M Dondorp,P Singhasivanon,N J White,N P J Day,

A simple and rapid liquid chromatographic-mass spectrometric assay for the evaluation of artesunate in vials for injection has been developed and validated. The content of each vial was dissolved in 3.0 mL of methanol using a SGE analytical syringe (1.0 mL). Each sample was diluted to a theoretical concentration of 1000 ng/mL and analysed in triplicate. Three replicates of calibration standards at concentrations 500, 1000 and 1500 ng/mL were used to construct a calibration curve. Artesunate was analysed by liquid chromatography with atmospheric pressure chemical ionisation (APCI) mass spectrometric (MS) detection on a Hypersil Gold column (100 mm x 4.6 mm) using a mobile phase containing methanol-ammonium acetate 10 mM pH 5.3 (70:30, v/v) at a flow rate of 1 mL/min. The assay was implemented for the analysis of artesunate for injection purchased from Guilin Pharmaceutical Company in China.

Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Publishing Authors By Initials

N ,AM Dondorp,P Singhasivanon,NJ White,NP Day,

For similar organic chemicals: hydrocarbons: terpenes: sesquiterpenes research abstracts see: organic chemicals: hydrocarbons: terpenes: sesquiterpenes research

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Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 45

Page Numbers: 149-53

Journal Abbreviation:

ISSN: 0731-7085

DAY: 29

MONTH: 04

YEAR: 2007

Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8309336

Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Keywords Mesh Terms:

KEYWORDS: Sesquiterpenes

MESH TERMS: analysis

Chemical & Substance for Abstract: Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples. Information

Substance Name: artesunate

Registry Number: 83507-69-1

Grant and Affiliation Information for Validation and application of a liquid chromatographic-mass spectrometric method for determination of artesunate in pharmaceutical samples.

AFFILIATION: Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. niklas@tropmedres.ac

Country: England

AGENCY: United Kingdom Wellcome T

GRANT: 077166/Z/05/Z

ACRONYM:

MEDLINETA: J Pharm Biomed Anal

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