V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells.

V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells. Research Abstract Details 

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V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells. Abstract Text:

Lu Lu,Yun Zhu,Junchen Diao,Zuguang Wang,Ying-Hua Chen,Lu Lu,Yun Zhu,Junchen Diao,Zuguang Wang,Ying-Hua Chen,

Previous studies have found the close correlation between epitope density and epitope-specific response, which have shown that high epitope density in a single recombinant protein molecule significantly enhances the humoral response and protective immunity. However, it has not been determined whether this kind of high epitope density could also significantly influence T cell response. Based on this, a series of recombinant DNA and proteins were designed and prepared. Each molecule consists of various copy numbers of the V3 CTL epitope on HIV-1 gp120 (one, two, four and eight copies). Our results show clearly that different V3-epitope densities in just one single DNA or protein molecules have respectively different effects on the number and activity of both primary and memory T cells. Interestingly, this effect is more complex than that on the B cells: epitope density in one plasmid or protein antigen affects the number, not the cytotoxic avidity, of primary CD8(+) T cells, but affects both the number and cytotoxic avidity of memory CD8(+) T cells. It indicates epitope density in the antigen is an important consideration to optimize T cell response induction and may facilitate the development of effective T cell-based anti-virus vaccines.

V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells. Publishing Authors By Initials

L Lu,Y Zhu,J Diao,Z Wang,YH Chen,L Lu,Y Zhu,J Diao,Z Wang,YH Chen,

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V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Vaccine

VOLUME: 26

Page Numbers: 845-52

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 29

MONTH: 11

YEAR: 2007

V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells. Information

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LANGUAGE: eng

NlmUniqueID: 8406899

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Grant and Affiliation Information for V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number and activity of primary and memory CD8(+) T cells.

AFFILIATION: Laboratory of Immunology, Department of Biology and Institute of Biomedical Science, Tsinghua University, Protein Science Laboratory of the MOE, Beijing 100084, PR China.

Country: Netherlands

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MEDLINETA: Vaccine

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