V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin.

V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin. Research Abstract Details 

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V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin. Abstract Text:

Wei Zhang,Dong Wang,Elzi Volk,Hugo J Bellen,Peter Robin Hiesinger,Florante A Quiocho,Wei Zhang,Dong Wang,Elzi Volk,Hugo J Bellen,Peter Robin Hiesinger,Florante A Quiocho,

The V(0) complex forms the proteolipid pore of a vesicular ATPase that acidifies vesicles. In addition, an independent function in membrane fusion has been suggested in vacuolar fusion in yeast and synaptic vesicle exocytosis in fly neurons. Evidence for a direct role in secretion has also recently been presented in mouse and worm. The molecular mechanisms of how the V(0) components might act or are regulated are largely unknown. Here we report the identification and characterization of a calmodulin-binding site in the large cytosolic N-terminal region of the Drosophila protein V100, the neuron-specific V(0) subunit a1. V100 forms a tight complex with calmodulin in a Ca(2+)-dependent manner. Mutations in the calmodulin-binding site in Drosophila lead to a loss of calmodulin recruitment to synapses. Neuronal expression of a calmodulin-binding deficient V100 uncovers an incomplete rescue at low levels and cellular toxicity at high levels. Our results suggest a vesicular ATPase V(0)-dependent function of calmodulin at synapses.

V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin. Publishing Authors By Initials

W Zhang,D Wang,E Volk,HJ Bellen,PR Hiesinger,FA Quiocho,W Zhang,D Wang,E Volk,HJ Bellen,PR Hiesinger,FA Quiocho,

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V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 294-300

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 12

MONTH: 10

YEAR: 2007

V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for V-ATPase V0 Sector Subunit a1 in Neurons Is a Target of Calmodulin.

AFFILIATION: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Departments of Molecular and Human Genetics and Neuroscience and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.

Country: United States

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MEDLINETA: J Biol Chem

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