Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings.

Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Research Abstract Details 

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Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Abstract Text:

Rebecca L R Powell,Mateusz M Urbanski,Sherri Burda,Aubin Nanfack,Thompson Kinge,Phillipe N Nyambi,Rebecca L R Powell,Mateusz M Urbanski,Sherri Burda,Aubin Nanfack,Thompson Kinge,Phillipe N Nyambi,

The predominance of unique recombinant forms (URFs) of HIV-1 in Cameroon suggests that dual infection, the concomitant or sequential infection with genetically distinct HIV-1 strains, occurs frequently in this region; yet, identifying dual infection among large HIV cohorts in local, resource-limited settings is uncommon, since this generally relies on labor-intensive and costly sequencing methods. Consequently, there is a need to develop an effective, cost-efficient method appropriate to the developing world to identify these infections. In the present study, the heteroduplex assay (HDA) was used to verify dual or single infection status, as shown by traditional sequence analysis, for 15 longitudinally sampled study subjects from Cameroon. Heteroduplex formation, indicative of a dual infection, was identified for all five study subjects shown by sequence analysis to be dually infected. Conversely, heteroduplex formation was not detectable for all 10 HDA reactions of the singly infected study subjects. These results suggest that the HDA is a simple yet powerful and inexpensive tool for the detection of both intersubtype and intrasubtype dual infections, and that the HDA harbors significant potential for reliable, high-throughput screening for dual infection. As these infections and the recombinants they generate facilitate leaps in HIV-1 evolution, and may present major challenges for treatment and vaccine design, this assay will be critical for monitoring the continuing pandemic in regions of the world where HIV-1 viral diversity is broad.

Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Publishing Authors By Initials

RL Powell,MM Urbanski,S Burda,A Nanfack,T Kinge,PN Nyambi,RL Powell,MM Urbanski,S Burda,A Nanfack,T Kinge,PN Nyambi,

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Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: AIDS research and human retroviruses

VOLUME: 24

Page Numbers: 100-5

Journal Abbreviation: AIDS Res. Hum. Retroviruses

ISSN: 0889-2229

DAY: 15

MONTH: Jan

YEAR: 2008

Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Information

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LANGUAGE: eng

NlmUniqueID: 8709376

Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings. Keywords Mesh Terms:

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AFFILIATION: Department of Microbiology, New York University School of Medicine, New York, New York 10010.

Country: United States

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MEDLINETA: AIDS Res Hum Retroviruses

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Utility of the Heteroduplex Assay HDA as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings Related Publications

• A heteroduplex assay for the rapid detection of dual Human Immunodeficiency Virus Type 1 infections.
• Circulating recombinant form (CRF) 37_cpx: an old strain in Cameroon composed of diverse, genetically distant lineages of subtypes A and G.
• Identification of a novel circulating recombinant form (CRF) 36_cpx in Cameroon that combines two CRFs (01_AE and 02_AG) with ancestral lineages of subtypes A and G.
• Quasispecies analysis of novel HIV-1 recombinants of subtypes A and G reveals no similarity to the mosaic structure of CRF02_AG.
• Utility of the Heteroduplex Assay (HDA) as a Simple and Cost-Effective Tool for the Identification of HIV Type 1 Dual Infections in Resource-Limited Settings.