Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays.

Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Research Abstract Details 

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Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Abstract Text:

Lu Charboneau,Heather Tory,Heather Scott,Tina Chen,Mary Winters,Emanuel F Petricoin,Lance A Liotta,Cloud P Paweletz,

Protein microarrays offer a new means by which to conduct quantitative profiling of disease-associated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of proteins potentially linked to disease aetiology and progression. The challenge is to evaluate quantitatively promising lead protein candidates using matched normal and diseased cell populations. In contrast to the antibody array, the reverse phase protein microarrays (RPPA) do not require labelling of cellular protein lysates, and constitute a sensitive high throughput platform for marker screening, pathophysiology investigation and therapeutic monitoring. In this paper, examples will be provided using RPPAs in the study of the apoptotic signalling cascade and in the evaluation of the expression of organ-specific protein makers using microdissected human organ cell lysates configured as 'human body arrays'.

Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Publishing Authors By Initials

L Charboneau,H Tory,H Scott,T Chen,M Winters,EF Petricoin,LA Liotta,CP Paweletz,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research

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Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Journal Published:

PUBLICATION TYPE: Review

Journal: Briefings in functional genomics & proteomics

VOLUME: 1

Page Numbers: 305-15

Journal Abbreviation:

ISSN: 1473-9550

DAY: 21

MONTH: Oct

YEAR: 2002

Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Information

Number of References: 25

LANGUAGE: eng

NlmUniqueID: 101150306

Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Keywords Mesh Terms:

KEYWORDS: Signal Transduction

MESH TERMS: metabolism

Chemical & Substance for Abstract: Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Utility of reverse phase protein arrays: applications to signalling pathways and human body arrays.

AFFILIATION: Laser Capture Microdissection Core Facility, and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Country: England

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MEDLINETA: Brief Funct Genomic Proteomic

REFSOURCE: Brief Funct Genomic Proteomic. 2007 Jun;

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