Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition.

Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Research Abstract Details 

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Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Abstract Text:

Jeffrey A Ranish,Marjorie Brand,Ruedi Aebersold,

One of the primary goals of proteomics is the description of the composition, dynamics, and connections of the multiprotein modules that catalyze a wide range of biological functions in cells. Mass spectrometry (MS) has proven to be an extremely powerful tool for characterizing the composition of purified complexes. However, because MS is not a quantitative technique, the usefulness of the data is limited. For example, without quantitative measurements, it is difficult to detect dynamic changes in complex composition, and it can be difficult to distinguish bona fide complex components from nonspecifically copurifying proteins. In this chapter, we describe a strategy for characterizing the composition of protein complexes and their dynamic changes in composition by combining affinity purification approaches with stable isotope tagging and MS. The use of software tools for statistical analysis of the data is also described.

Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Publishing Authors By Initials

JA Ranish,M Brand,R Aebersold,

For similar fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research abstracts see: fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research

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Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 359

Page Numbers: 17-35

Journal Abbreviation:

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: metabolism

Chemical & Substance for Abstract: Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition.

AFFILIATION: Institute for Systems Biology, Seattle, WA, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR11823

ACRONYM: RR

MEDLINETA: Methods Mol Biol

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