Using genomic microarrays to study insertional/transposon mutant libraries.

Using genomic microarrays to study insertional/transposon mutant libraries. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Using genomic microarrays to study insertional/transposon mutant libraries. Abstract Text:

David N Baldwin,Nina R Salama,

The rapid expanse of microbial genome databases provides incentive and opportunity to study organismal behavior at the whole-genome level. While many newly sequenced genes are assigned names based on homology to previously characterized genes, many putative open reading frames remain to be annotated. The use of microarrays enables functional characterization of the entire genome with respect to genes important for different growth conditions including nutrient deprivation, stress responses, and virulence. The methods described here combine advancements in the identification of genomic sequences flanking insertional mutants with microarray methodology. The combination of these methods facilitates tracking large numbers of mutants for phenotypic studies. This improves both the efficiency of genome-saturating library screens and contributes to the functional annotation of unknown genes.

Using genomic microarrays to study insertional/transposon mutant libraries. Publishing Authors By Initials

DN Baldwin,NR Salama,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: oligonucleotide array sequence analysis research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: oligonucleotide array sequence analysis research

PUBMED ID PMID:

MEDLINE DATE:

Using genomic microarrays to study insertional/transposon mutant libraries. Journal Published:

PUBLICATION TYPE: Review

Journal: Methods in enzymology

VOLUME: 421

Page Numbers: 90-110

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2007

Using genomic microarrays to study insertional/transposon mutant libraries. Information

Number of References: 18

LANGUAGE: eng

NlmUniqueID: 212271

Using genomic microarrays to study insertional/transposon mutant libraries. Keywords Mesh Terms:

KEYWORDS: Oligonucleotide Array Sequence Analysis

MESH TERMS:

Chemical & Substance for Abstract: Using genomic microarrays to study insertional/transposon mutant libraries. Information

Substance Name: DNA Transposable Elements

Registry Number: 0

Grant and Affiliation Information for Using genomic microarrays to study insertional/transposon mutant libraries.

AFFILIATION: Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI 054423

ACRONYM: AI

MEDLINETA: Methods Enzymol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Using genomic microarrays to study insertional/transposon mutant libraries Related Publications

• A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer.
• A rapid gel agglutination test for the determination of fetomaternal haemorrhage.
• Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.
• Application of the particle gel agglutination system as a new check gel assay for PCR products.
• Association between alloantibody specificity and autoantibodies to red blood cells.
• Autoimmune thrombocytopenia in two patients with Sneddon's Syndrome.
• Autonomic nerve stimulation reverses ventricular repolarization sequence in rabbit hearts.
• Calcium transients modulate action potential repolarizations in ventricular fibrillation.
• Design optimization methodology for dry EEG spikes array electrode.
• Dreams and dreaming. Part one.
• Dreams and dreaming. Part two.
• Dual-dye optical mapping after myocardial infarction: does the site of ventricular stimulation alter the properties of electrical propagation?
• Effects of marjoram volatile oil and grape seed extract on ethanol toxicity in male rats.
• Enhanced aqueous solubility of long wavelength voltage-sensitive dyes by covalent attachment of polyethylene glycol.
• Hughes-Stovin syndrome: is it incomplete Behçet's? Report of two cases and review of the literature.
• Identification of Helicobacter pylori genes that contribute to stomach colonization.
• Identification of a novel HLA-DQB1 allele, HLA-DQB1*0632.
• Immune tolerance induction in patients with IgA anaphylactoid reactions following long-term intravenous IgG treatment.
• Iridoid Glucosides from Euphrasia rostkoviana.
• Monte Carlo Simulation of 3D Mapping of Cardiac Electrical Activity with Spinning Slit Confocal Optics.
• Occurrence and overlap of natural disasters, complex emergencies and epidemics during the past decade (1995-2004).
• Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation.
• Production of proinflammatory cytokines: granulocyte-macrophage colony stimulating factor, interleukin-8 and interleukin-6 by peripheral blood mononuclear cells of protein energy malnourished children.
• Ridge preservation and augmentation using regenerative materials to enhance implant predictability and esthetics.
• Sleep, physiology and disorders.
• Spatially discordant voltage alternans cause wavebreaks in ventricular fibrillation.
• Studies on the mechanism of action of sulfanilamide on mycelial felts of Rhizoctonia solani.
• Thyroid disorders and autoantibodies in systemic lupus erythematosus and rheumatoid arthritis patients.
• Using genomic microarrays to study insertional/transposon mutant libraries.
• [Aquired immune hemolytic anemias]