Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths.

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Abstract Text:

Jason Jonghyuk Lee,Anthony Nettey-Marbell,Archie Cook,Luiz A F Pimenta,Ralph Leonard, Ritter,Jason Jonghyuk Lee,Anthony Nettey-Marbell,Archie Cook,Luiz A F Pimenta,Ralph Leonard, Ritter,Jason Jonghyuk Lee,Anthony Nettey-Marbell,Archie Cook,Luiz A F Pimenta,Ralph Leonard, Ritter,

BACKGROUND: The Centers for Disease Control and Prevention has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. The authors conducted a study to test the null hypothesis that the storage medium and sterilization method have no effect on composite-to-dentin bond strengths. MATERIALS AND METHODS: The authors collected 170 bovine incisors, cleaned them and placed them randomly into one of six storage media at 37 degrees C for 60 days: distilled water (dH(2)O), 0.9 percent sodium chloride, 0.5 percent chloramine-T, 5.25 percent sodium hypochlorite (NaClO), 2 percent glutaraldehyde and 10 percent formalin. For sterilization, they autoclaved a subset of 10 specimens from every sample, while they stored another subset of 10 specimens from every sample (except for the 10 percent formalin sample) in 10 percent formalin for 14 days. The authors then embedded the specimens in epoxy and ground flat the facial surface to expose middle-depth dentin, which they polished to 600 grit. They used a dental adhesive to apply composite to the exposed dentin. The authors tested the composite-to-dentin shear bond strength 24 hours after bonding. They analyzed the data using global analysis of variance and, when appropriate, multiple post hoc tests (P = .05). RESULTS: Storage in NaClO resulted in significantly lower bond strength than that of the other treatment specimens. Sterilization with the autoclave negatively affected the bond strength of specimens stored initially in dH(2)O or 10 percent formalin, while sterilization with formalin alone had no significant effect on bond strengths. CONCLUSIONS: Storing bovine teeth in 5.25 percent NaClO may negatively affect composite-to-dentin bond strengths. Immersion in 10 percent formalin might be the best option for storage and sterilization of bovine teeth that are to be used in dental bonding studies in vitro.

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Publishing Authors By Initials

JJ Lee,A Nettey-Marbell,A Cook,LA Pimenta,R Leonard,AV Ritter,JJ Lee,A Nettey-Marbell,A Cook,LA Pimenta,R Leonard,AV Ritter,JJ Lee,A Nettey-Marbell,A Cook,LA Pimenta,R Leonard,AV Ritter,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of the American Dental Association (1939)

VOLUME: 138

Page Numbers: 1599-603

Journal Abbreviation:

ISSN: 0002-8177

DAY: 6

MONTH: Dec

YEAR: 2007

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503060

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths.

AFFILIATION: School of Dentistry, University of North Carolina at Chapel Hill, NC 27599, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Dent Assoc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths Related Publications

• A method for cultivating Hemophilus influenzae.
• A novel method for the preparation of dimethylneopentylcarbinol.
• An alternative method to estimate zero flow temperature differences for Granier's thermal dissipation technique.
• Applied cognitive science laboratory at the pennsylvania state university.
• Cell volume regulatory ion transport in the regulation of cell migration.
• Characterization of rat intestinal microsomal UDP-glucuronosyltransferase activity toward mycophenolic acid.
• Cytogenetic effects of low-dose radiation with different LET in human peripheral blood lymphocytes.
• Development of auditory selective attention: event-related potential measures of channel selection and target detection.
• Direct Pulp-Capping Performed by Dental Students has a Success Rate Close to 60%. Direct Capping of Carious Pulp Exposures is Significantly Less Successful (33%) than Direct Capping of Mechanical Pulp Exposures (92%).
• Effects of dietary supplementation with L-carnitine and fat on blood acid-base responses to handling in slaughter weight pigs.
• Halitosis.
• Interrelation amongst differentiation, senescence and genetic instability in long-term cultures of fibroblasts exposed to different radiation qualities.
• Is there a functional benefit to obtaining high flexion after total knee arthroplasty?
• Isolated fracture of the atlas; report of two cases.
• Leptin analog antagonizes leptin effects on food intake and body weight but mimics leptin-induced vagal afferent activation.
• Localized glucoprivation of hindbrain sites elicits corticosterone and glucagon secretion.
• Positional plagiocephaly: evaluation and management.
• Protein appetite is increased after central leptin-induced fat depletion.
• Revised Fuchsin Lactose Broth for the Confirmatory Test in Water Analysis.
• Salvage hypofractionated radiotherapy for biochemically recurrent prostate cancer after radical prostatectomy.
• Studies of the Toxicity of Basic Fuchsin for Certain Bacteria.
• The indications and technic of foot stabilization in infantile paralysis.
• The influence of inducible costimulator fusion protein (ICOSIg) gene transfer on corneal allograft survival.
• The technique of electrocardiography.
• Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths.
• [Increase of cholesterol elimination in the duodenum by phenylethylacetic acid.]
• [Mobile single-pass batch hemodialysis system in intensive care medicine. Reduction of costs and workload in renal replacement therapy]
• [Not Available.]
• [Not Available.]
• [Not Available.]